Fig. 6. Fluorescein diacetate staining in the regenerated sub-protoplast. (A-D) Confocal laser scanning micrographs of the sub-protoplast regeneration taken at various times post-wounding. Artificial colours indicate an increase of fluorescence intensity inside the sub-protoplast over time. (A'-D') Quantitative analysis of the fluorescence intensities, measured along a line through the central portion of the sub-protoplast (D, distance; Sfi, subtracted fluorescence intensity). Bar, 10 µm.