Fig. 10. The primase subunit p50 is detected in the complex immunoprecipitated by anti-Dpit47 antibody. Dpit47 immunoprecipitates as described in Materials and Methods were analysed using anti-p180 (polp180) and anti-p50 (polp50) antibodies. In each case two seperate experiments are shown. The lane after the ce contains the last wash of the immunoprecipitate prior to elution to show that there is no carryover between fractions. 250 mM and 500 mM, the 250 mM and 500 mM NaCl elutions from the immunoprecipitate, respectively; ce, crude extract as a control.