JCS -- Crevel et al. 114 (11): 2015 Figure IG11

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Fig. 11. The DNA polymerase ${alpha}$ complexed with Dpit47 is inactive. DNA polymerase ${alpha}$ was immunoprecipitated using anti-Dpit47 antibody or monoclonal anti-DNA polymerase ${alpha}$ antibody. Top panel, DNA polymerase assay: immunoprecipitated DNA polymerase ${alpha}$ was used to carry out an end filling polymerase assay on a 35 mer as described in Materials and Methods. The reaction was stopped after 5, 15 or 30 minutes. The product of the reaction was analysed on a 15% polyacrylamide gel. Lower panel shows by western blotting the amount of DNA polymerase ${alpha}$ contained in the Dpit47 (Dpit47 IP) and DNA polymerase ${alpha}$ (pol ${alpha}$ IP) immunoprecipitates used in the assay.