Fig. 2. Co-immunoprecipitation of Dpit47 and DNA polymerase . Drosophila embryo crude extract was subjected to immunoprecipitation using a polyclonal antibody against Dpit47 cross-linked to protein A sepharose. The immunocomplexes were washed extensively and then eluted with increasing salt concentrations and SDS as indicated in the figure. The top panel represents the elution profile of DNA polymerase (polp180), and the bottom represents that of the Dpit47 in the same experiment. c, control immunoprecipitation using an unrelated antibody. 1 and 2, immunoprecipitation using anti-Dpit47 antibody (duplicate experiments are shown for each immunoprecipitate).