Fig. 6. DNA polymerase is associated with Dpit47 throughout development and in proliferating cell culture but not in quiescent cells. (a) A comparison of the amount of DNA polymerase associated with Dpit47 complexes immunoprecipitated at different stages of the life cycle from 0 to 24 hours as indicated. The amounts of DNA polymerase in each extract is indicated (loading controls). In each case equal amounts of the immunoprecipitated Dpit47 was loaded as determined by western blotting. (b) A comparison of the amount of DNA polymerase associated with Dpit47 complexes immunoprecipitated from Drosophila embryo extract (E) and exponentially growing S2 cells (C). The two panels on the left are western blots showing the amounts of DNA polymerase and Dpit47 in crude extracts from Drosophila embryos and S2 cells. The panel on the top right shows the amount of DNA polymerase (as determined by western blot) immunoprecipitated with anti-Dpit47 antibody from each extract. The bottom right panel has been included to show that equal amounts of Dpit47 (as analysed by coomassie staining) were analysed for each extract. (c) A comparison of the amount of DNA polymerase associated with Dpit47 complexes immunoprecipitated from exponentially growing (D) or quiescent (Q) S2 cells. The two panels on the left are western blots showing the amounts of DNA polymerase and Dpit47 in crude extracts from exponentially growing cells and quiescent cells. The panel on the top right shows the amount of DNA polymerase (western blot) immunoprecipitated with anti Dpit47 antibody for each extract. As above the bottom right panel has been included to show that equal amounts of Dpit47 immunoprecipitate have been compared for each cell type (in this case by western blot).