Fig. 8. Cellular fractionation of Dpit47 and DNA polymerase . Drosophila embryo extracts were subjected to salt and detergent fractionation and analysed by western blot using anti Dpit47 and anti-DNA polymerase (pol) antibodies as described in Materials and Methods. Ce, total cellular extract as a control; 50 mM NaCl, cytoplasmic fraction; Triton, protein released on treatment of nucleus with triton, representing the nucleoplasmic fraction; Triton final, last triton wash before salt extractions to show that there is no carry over between fractions; 250 mM NaCl, Triton pellet extracted with 250 mM NaCl and 1% Triton; pellet, remainder after all extractions.