Fig. 9. Analysis of Dpit47 DNA polymerase complexes in the cytoplasm and in the nucleoplasm. Cytoplasmic and nucleoplasmic fraction were subjected to immunoprecipitations using anti-Dpit47 antibody. The DNA polymerase eluted in 250 mM NaCl from two separate experiments fractions was determined by western blots using anti-DNA polymerase antibody. For the cytoplasmic fraction (top panel) the lane ce on the left corresponds to shorter exposure time in order to visualise the band. The same exposure time as the eluted fractions is shown on the right. i and ii, two separate elutions; ce, crude extract control.