Fig. 2. Formation of morphogenetic structures by various HB2 cell populations. The structures were designated ball-like, cyst shaped, and branching, as described in Materials and Methods. The micrographs show the structures formed by control cells (A), Wnt-5a-overexpressing cells (B) and Wnt-5a antisense cells (C). One hundred morphogenetic structures of each cell population were examined and classified on each of the triplicate plates. Mean values were calculated and plotted on the graph (D). The values represent the mean and standard deviation of determinations and are expressed as percent of the scored structures. Bar, 80 µm.