Fig. 5. The cell-to-collagen binding ability of various cell populations. (A) The data represent the average number of cells that adhered to a matrix containing various concentrations of collagen type I during 2 hours incubation. The values are given as the mean and standard deviation of determinations done on triplicate wells assayed in parallel. (B) Tyrosine phosphorylation of FAK in cells with different Wnt-5a protein levels. Upper panel: level of FAK expression in Wnt-5a-overexpressing cells (lane 1), in antisense cells (lane 2), and in control cells (lane 3). Lower panel: level of FAK phosphorylation in various cell populations as indicated in B. The molecular weight of FAK was estimated to 125 kDa by comparing with a molecular standard weight.