Fig. 9. Effects of ß-catenin accumulation on DDR1 phosphorylation. (A) The presence of secreted Wg protein in medium conditioned by S2HS-Wg compared to that conditioned by S2 control cells. (B) Extracts from various cell populations, grown on collagen for 30 minutes in the presence of Wg protein, were prepared and analysed for phosphorylation of DDR1 receptors. Double extracts were also prepared in parallel to detect induction of ß-catenin protein. (C) Accumulation of ß-catenin in response to incubation with concentrated Wg-conditioned medium, ensured by exposing cells to medium conditioned for 30 minutes by control S2 cells (lanes 1-3) or Wg-producing cells (lanes 4-6).