Fig. 8. Codistribution of F-actin and plectin N terminus protein fragments in transfected cells. Rat embryo fibroblasts were transiently transfected with HA-tagged plectin_1-339 (A-C) or HA-tagged plectin_65-339 (D-F). The cells were fixed, permeabilized and processed for double labeling using rhodamine-phalloidin to visualize F-actin (A,D) and anti-HA antibodies, followed by FITC-conjugated secondary antibodies to detect the HA-tagged proteins (B,E). Images were obtained using a Leica confocal microscope. (C,F) Composite images. Colocalization appears as yellow. Bar, 10 µm.