Fig. 3. Double immunofluorescence of normal and lesional skin with Cx26 (green) and Cx43 (red) antibodies. (A) Normal plantar skin. (B) Lesional plantar skin of II-1 (carrier of E42). Areas of focal co-localization of Cx26 and Cx43 are shown in yellow. (C) Lesional skin of the lateral foot of III-2 (compound heterozygous for E42/35delG). Normal expression of Cx26 by the epithelium of an eccrine sweat duct with punctate staining of the plasma membranes. Note the focal co-localization of Cx26 and Cx43 in suprabasal keratinocytes surrounding the sweat duct. (D) Normal-appearing skin (arm) of II-2 (homozygous 35delG mutation carrier) with absent immunostaining of Cx26. (E) Normal-appearing skin (arm) of II-1 (carrier of E42) demonstrating basal expression of Cx26 with extension into the lower suprabasal cells which results occasionally in co-localization of Cx26 and Cx43. (F) Viral wart. Epidermal hyperproliferation is associated with strong expression of Cx26 throughout the upper epidermis and extensive co-localization of Cx26 and Cx43.e, epidermis; d, dermis; sd, sweat duct. Bar, 40 µm (A,B,C,F); 24 µm (D); 120 µm (E).