Fig. 6. FSH and MMP-2 gene expression. Sertoli cells were treated or not (control (c)) with FSH (50 ng ml^-1) or 8-bromo-AMP (10^-3 M) for 48 hours, after which RNA was extracted and 3 µg of PolyA(+) were analyzed by northern blot (A). Membranes were successively probed with MMP-2 and GAPDH. A representative blot is shown in the inset (lane 1, control; lane 2, FSH; lane 3, 8-bromo-cAMP). Data yielded by scanning three independent blots were normalized to the GAPDH signal and expressed as mean±s.e.m. of three dishes. P values against the control: *, 0.05; **, 0.01. (B) MMP-2 antigen levels were analyzed by western blot in the 72-hour culture media of Sertoli cells treated with or without FSH.