Fig. 7. FSH and MT1-MMP gene expression. Sertoli cells were treated or not (control (c)) with FSH (50 ng ml^-1) or 8-bromo-AMP (10^-3 M) for 48 hours, after which RNA was extracted and 3 µg of polyA(+) were analyzed by northern blot (A). Membranes were successively probed with MT1-MMP and GAPDH. A representative blot is shown. (B) MT1-MMP antigen levels were analyzed by western blot in the lysates of Sertoli cells treated with FSH (+) or not (-) for 24, 48 or 72 hours.