(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)


Fig. 6. Cdc13 cyclin is stabilized in the mfr1{Delta}/mfr1{Delta} mutant. (A) Strains h-/h- pat1-114/pat1-114 (Sp964) and h-/h-pat1-114/pat1-114 mfr1{Delta}/mfr1{Delta} (Sp965) were synchronized through meiosis as indicated in Fig. 2. Samples were taken every 30 minutes and protein extracts were analyzed by western blot with antibodies to cig1, cdc13 and cdc2. Exp, exponentially growing cells before nitrogen starvation. (B) Cdc2 protein kinase assays after immunoprecipitation with cdc2 antibodies. Samples were taken every hour.