Fig. 3. Nomarski contrast (left column) and immunofluorescence (right column) images of control cells (A,B,E,F) and quinine-treated cells (C,D,G,H) stained with an antibody against the 100 kDa subunit of V-ATPase (A,B,C,D) or an anti-calmodulin antibody (E,F,G,H). Cells were fixed after a 5 minute incubation with 2 mM quinine. Insets in C and D show an enlarged contractile vacuole in a quinine-treated cell. Bar, 5 µm.