Fig. 1. Colocalization of the EGFR and c-Cbl with clathrin in stimulated cells. Serum starved COS-7 cells transiently expressing human c-Cbl were incubated without (A,C) or with (B,D) 25 ng ml^-1 EGF for 5 minutes at 37°C. Prior to fixation, cells were permeabilized with saponin. Cells were incubated with antibody to the EGFR (polyclonal antibody RK-2) (A,B) or c-Cbl (C,D), followed by Texas-Red-conjugated secondary antibody and double stained with an antibody to clathrin (mAb 23) followed by FITC-conjugated anti-mouse Ig. Merged confocal images of the EGFR or c-Cbl (red) and clathrin (green) are shown.