Fig. 8. Immuno-electron microscopy reveals c-Cbl in multivesicular bodies. COS-7 cells transfected with c-cbl cDNA were stimulated with EGF for 15 minutes (A,B) or 60 minutes at 37°C (C). Ultrathin cryosections were single labelled to detect EGFR (A) or c-Cbl (B), or double labelled to detect both EGFR and c-Cbl (C). Abbreviations: c-Cbl¹⁰, c-Cbl 10 nm gold particle; EGFR¹⁵, EGFR 15 nm gold particle. Bar, 100 nm.