Fig. 2. SHP-2 forms a complex with a p120 tyrosyl-phosphorylated protein during C2C12 myogenesis. C2C12 myoblasts cultured at the indicated times were subjected to (A) immunoprecipitation (IP) with either preimmune (PI) antisera or anti-SHP-2 antibodies. Immune complexes were resolved by SDS-PAGE, transferred to Immobilon and immunoblotted with anti-phosphotyrosine (pTyr) antibodies. The arrows to the right indicate the positions of the p120 and p180 tyrosyl-phosphorylated proteins that associate with SHP-2 during differentiation. The immunoblot was reprobed with anti-SHP-2 antibodies (bottom). (B) Cell lysates from C2C12 myoblasts cultured as indicated were immunoprecipitated with anti-SHP-2 antibodies and immunoblotted with pTyr antibodies. As a control, this immunoblot was reprobed with anti-SHP-2 antibodies (bottom). The positions of molecular mass markers are shown.