Fig. 8. Insulin, IGF-I and IGF-II fail to induce SHPS-1 tyrosyl phosphorylation. C2C12 myoblasts were either left unstimulated, or were stimulated with insulin, IGF-I or IGF-II following serum deprivation for 6 hours. Cell lysates prepared from C2C12 myoblasts following stimulation with the indicated growth factor were immunoblotted with anti-phospho MAPK and reprobed with anti-MAPK antibodies (top). These cell lysates were also immunoblotted with anti-phospho-Akt antibodies and reprobed with anti-Akt antibodies (middle). These immunoblots were also reprobed with anti-Akt antibodies. SHPS-1 immunoprecipitated from the cell lysates shown in the top panels were immunoblotted with either anti-pTyr or anti-SHPS-1 antibodies (bottom).