Fig. 10. Quantitation of the extent of colocalisation between Hrs constructs and EEA1. BHK cells were transfected with the indicated myc-tagged Hrs constructs and permeabilised with saponin prior to fixation and immunolabelling with anti-EEA1 and anti-myc antibodies. Images of randomly selected transfected cells were recorded by confocal microscopy. Profiles positive for both EEA1 and the respective Hrs construct were counted manually, and these numbers were expressed as a percentage of the total number of EEA1-positive structures. For each Hrs construct, 20 cells (10 cells each from two independent transfections) were analysed. In total, about 12,000 profiles were counted in this analysis. Values are means ± s.e.m.