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Fig. 4. Colocalisation of Hrs constructs with EEA1. BHK cells were transfected with myc-tagged Hrs (A), Hrs{Delta}VHS (B), HrsFYVE (C), Hrs1-500 (D), Hrs1-573 (E) or Hrs500-775 (F) and stained with anti-myc (left) or anti-EEA1 (middle). (Right) The merged images, with yellow colour indicating colocalisation. Nuclei are visualised with Toto-3 (blue colour). Bar, 5 µm.