JCS -- Raiborg et al. 114 (12): 2255 Figure IG6

(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)

Fig. 6. Biophysical and biochemical properties of wild-type and mutant Hrs FYVE domains. The ${alpha}$ -helical content (in millidegrees) of GST-fusions of wild-type and mutant FYVE domains was studied by CD spectroscopy (A), and the binding of GST-Hrs_FYVE and GST-Hrs_FYVE^R183A to PtdIns(3)P was analysed by surface plasmon resonance (B), as described in Materials and Methods. RU, resonance units.