Fig. 9. Colocalisation of Hrs constructs with endocytosed Alexa⁴⁸⁸-transferrin. BHK cells were cotransfected with the human transferrin receptor and myc-tagged Hrs (A) or with Hrs_FYVE+CC2 (B) and incubated with Alexa⁴⁸⁸-transferrin at 37°C for 15 minutes. The fixed cells were stained with anti-myc antibodies (left); (middle) the internalized Alexa⁴⁸⁸-transferrin; (right) the merged images. Yellow colour indicates colocalisation. Bar, 5 µm.