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Fig. 7. SSeCKS is enriched in the insoluble fractions of cell lysates. Total cell lysate (T), soluble fraction (S) and insoluble pellet (I) were resolved by 6% PAGE and subsequently immunoblotted with polyclonal SSeCKS antibody. To verify the fractionation of soluble and insoluble fractions, parallel assays were probed with antibodies against E-cadherin.