Fig. 8. Expressing the cytoplasmic and transmembrane domains of GalT (TL) creates a cell spreading defect that is reversed by co-expression of GFP-1.12. (A) nontransfected NMuMG cells. (B) NMuMG cells stably transfected with TL-GFP. (C) NMuMG cells stably transfected with both TL-GFP and GFP-1.12. (D) NMuMG cells stably transfected with both TL-GFP and empty BFP vector. Representative lysates of 1.12 and TL-GFP double transfectants (clones 3 and 8) were immunoprecipitated with anti-GFP antibodies and stained for GFP fusion proteins using polyclonal anti-GFP (E) to confirm the expression of the 1.12 fusion protein.