Fig. 5. NG2-mediated cell polarization. B28 cells transfected with wild-type NG2 were serum-starved overnight, resuspended in DMEM/BSA for 1 hour, and then allowed to spread for 8 hours in DMEM/FCS on plates coated with mAb D120. Cells were then fixed and double-stained with rabbit anti-NG2 (a) and mouse monoclonal anti-ß-actin (b), or with rabbit anti-B28 (c) and mouse monoclonal anti-fascin (d). Retraction fibers at one pole of the cell are strongly NG2-positive, whereas actin and fascin are localized to lamellipodia at the opposite pole. Bar, 10 µm (a).