Fig. 7. Effect of NG2 chondroitin sulfate chain on cell polarization. Cell polarization was examined after 8 hours on PLL plates in the presence of 1 µg/ml LPA (d-f) and on mAb D120 plates in DMEM/BSA (a-c). Under both sets of conditions, B28 cells transfected with wild-type NG2 (a,d) and with NG2/S1342A (b,e) exhibited a high tendency to polarize. By contrast, many fewer instances of polarization were observed for NG2/S999A-transfected cells (c,f). Bar, 10 µm (f).