Fig. 5. Exogenous CHMP1 locally condenses chromatin and alters histone modification. IND-CHMP1 cells were induced with muristerone (24 hours mur) or left uninduced (0 hours mur). (A) Cells were fixed, then triple-labeled with anti-CHMP1, Hoechst 33258 and propidium iodide (PI) or (B) double-labeled for DNA and antibody to modified forms of histone H3 (Upstate Biologicals). Note the strong staining only in the mitotic cell at 0 hours. Ac-H3, acetylated (Lys9,14); P-H3, phosphorylated (Ser10).