Fig. 8. CHMP1 produces a PcG phenotype upon misexpression in Xenopus. (A) Four-cell-stage embryos were unilaterally injected (arrowhead) with the indicated synthetic, capped RNA plus a marker RNA encoding ß-galactosidase and processed to measure the side of injection (in situ ß-galactosidase assay) and En-2 expression pattern (Yoshitake et al., 1999). (B) Embryos were injected with 0.1 ng of each indicated RNA. At stage 20 embryos were fixed and analyzed for En-2 expression by RNA in situ hybridization. The percentage of embryos with the indicated effects is the average of three independent experiments. Standard error is indicated. Control embryos show less than 3% repression and 0.5% shift.