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Fig. 3. A proline-rich region in the cytoplasmic tail of FasL is responsible for sorting to the secretory granules. FasLWT-GFP and FasL{Delta}pro-GFP (lacking the proline-rich region, amino acids 45-74; Fig. 2a) were stably expressed in RBL cells and analysed by confocal microscopy (a) and FACS analysis (b) as described in Fig. 2.