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Fig. 8. Clustering of centromeres in the cdc25 mutant zygotes during RTG. Number of cells with indicated number of centromere signals are plotted against the time of incubation. When centromere signals were in contact, they were regarded as one signal. The single centromere cluster was always observed at the SPB. 0 hours is the time point when the incubation temperature was shifted to 35°C.