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Fig. 6. Time dependence of (A) EGFR activation and (B) Shc translocation. Frequency histograms after 2, 5, 8 and 20 minute incubations of A431 cells with EGF-coated microspheres. Insets show mean receptor activation as well as Shc translocation expressed in arbitrary fluorescence units (a.u.). Values are means ± 1 s.d. of three independent experiments.