Fig. 7. The silver stain profile of proteins from phagosomes isolated from cells overexpressing DN Rab7 lacks bands found in phagosomes isolated from control cells. Phagosomes were purified as described in Materials and Methods from control cells and cells overexpressing DN Rab7 pulsed with latex beads for 30 minutes. Phagosomal proteins were separated by SDS-PAGE and silver stained as described in Materials and Methods. H, whole cell homogenate; X4, control phagosomes; DN7, phagosomes from cells overexpressing DN Rab7. Arrows denote bands missing from the DN Rab7 phagosome protein profile. The positions of marker proteins (kDa) are shown.