
Fig. 3. DNA fragmentation in L. donovani promastigotes with or without H2O2 treatment in culture as detected by TUNEL staining. (A) At 1 hour without H2O2 exposure. (B) At 1 hour with H2O2 treatment. (C) At 2 hours without H2O2 treatment. (D) At 2 hours after H2O2 treatment. (E) At 6 hours without H2O2 treatment. (F) At 6 hours after H2O2 treatment. (B1-F1) Scan-enhanced close ups of promastigotes with H2O2 treatment for 1 hour (B1), after 2 hours exposure to H2O2 (D1; notice the kinetoplast (k) staining) and after 6 hours exposure to H2O2 (F1; notice both kinetoplast and nuclei (n) staining). Bar, 10 µm.