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Fig. 4. Immunoreactivity of the RPB1 subunit in Xenopus embryos. Whole-cell lysates from A6 cells (A6), batches of ten eggs (Unfertilised) or embryos taken from the same female, maintained at 25°C and sampled at 4.5 (Pre-MBT), 6.5 (Post-MBT) or 8.5 (Gastrula) h.p.f. were analysed by western blot using the indicated monoclonal antibodies.