JCS -- Osborne et al. 114 (13): 2501 Figure IG9

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Fig. 9. PtdIns(4,5)P₂ associates with splicing complexes. Splicing reactions were carried out prior to immunoprecipitation. In these conditions, intermediates and products of the splicing reaction associate with anti-PtdIns(4,5)P₂, but not control beads. Pre-incubation of the antibody with GroPIns(4,5)P₂ but not GroPIns inhibits the recovery. A positive control immunoprecipitation with Y12 is shown for comparison.