Fig. 1. Comparison of the darkfield wave patterns during early aggregation. The left column A-E shows the actual wave patterns of the different wild-type and mutant strains at the same scale (Bar, 10 mm). All images were enhanced by image subtraction. The according time-space plots are displayed in the right column, F-J. They all cover a period of 67 minutes (200 images), except for N272 (J) with 133 minutes (400 images) to show the disappearance of the waves. The vertical axis of the time-space plots corresponds to the time axis, time increases from the top to the bottom. (A,F) Wild-type strain Ax3 with spiral waves. (B,G) Parental strain DH1 with concentric waves. (C,H) cAR1 mutant with concentric waves. (D,I) cAR3 mutant with spiral waves. (E,J) N272 mutant with concentric waves.