Fig. 5. Localization of ß1 in GD25 cells expressing site directed substitutions at S785. Cells were plated on fibronectin and double labeled with antibodies directed against vinculin and chicken ß1. There is no ß1 in untransfected GD25 cells. ß1 does not localize to focal adhesion sites in GD25-S785D2 cells. By contrast, ß1 in both GD25-S785S4 and GD25-S785M2 cells colocalizes with vinculin to these structures.