Fig. 10. Emerin is located in aggregates within the ER in HeLa cells transfected with GFP-delta 2+. The distribution of emerin was compared with the distribution of calreticulin in cells that were transiently transfected with GFP-delta 2+ by four-channel fluorescence. Transfected cells were stained with DAPI to reveal the distribution of DNA; with MANEM5 (Cy5) to reveal the distribution of emerin; and with rabbit anti-calreticulin (TRITC) to reveal the distribution of calreticulin within the ER. Images are displayed either as individual black and white panels or a four-colour merged image in which DAPI is displayed in blue, GFP-delta 2+ in green, calreticulin in red, and emerin in white. It should be noted that when calreticulin staining was omitted no bleed through between the Cy5 and the TRITC filters was observed. Bars, 10 µm.