Fig. 2. Comparison of emerin and lamin C distributions in SW13 and HeLa cells. The level of expression of different lamins was compared in HeLa (A) and SW13 cells (B) by immunoblotting. Nuclei isolated from 10⁶ cells were resolved on 10% SDS-PAGE, transferred to nitrocellulose and immunoblotted with mAbs JoL2 (lamin A/C), JoL4 (lamin A), LN43 (lamin B₂), rabbit polyclonal anti-lamin C or goat anti-lamin B₁ using a multi-blot apparatus. The distribution of lamin C (blocks C and D), lamin A (blocks C and D), emerin (blocks E and F) and LAP2ß (blocks E and F) in HeLa cells (blocks C and E) and SW13 cells (blocks D and F) were investigated by indirect immunofluorescence using polyclonal anti-lamin C, JoL4, MANEM5 and LAP17, respectively. In all samples the distribution of DNA was detected with DAPI. Bars, 10 µm.