Fig. 4. GFP-emerin forms aggregates within a sub-domain of the ER in SW13 cells. GFP-emerin was transiently expressed in SW13 cells (rows A-D) or HeLa cells (row E) and its distribution was compared with lamin C (row A), lamin B₁ (row B), lamin B₂ (row C) or calreticulin (row D, ER) after fixation. In each sample the distribution of DNA was revealed with DAPI. Images are displayed as individual black and white panels and as three-colour merged images. In merged images `yellow' indicates spectral overlap between red and green signals. Arrowheads (row D) indicate the position of emerin aggregates within the ER. Bars, 10 µm.