Fig. 5. The distribution of lamin C and emerin in lymphoblastoid cell lines. EBV-transformed lymphoblastoid cell lines were obtained from a donor control (row A) and from an AD EDMD patient with a single point mutation giving rise to an amino acid substitution at position T528K (row B). Each cell line was co-stained with rabbit anti-lamin C (Lamin C) and MANEM5 (emerin). The distribution of DNA in each sample was detected with DAPI. Panels show individual black and white micrographs or three-colour merged images (merge) in which the distribution of DAPI is shown in blue, lamin C in green and emerin in red/orange. In merged images, `yellow' indicates spectral overlap between red and green signals. Bar, 10 µm.