Fig. 9. The influence of GFP-Delta 2+ on the distribution of emerin and LAP2ß in HeLa cells. HeLa cells were transiently transfected with GFP-delta 2+ and the distributions of emerin (row A) and LAP2ß (row B) were compared in transfected and untransfected cells by immunofluorescence using MANEM5 (emerin) and LAP17 (LAP2). The distribution of DNA was detected with DAPI. Panel displays individual black and white micrographs or three-colour merged images in which DAPI is shown in blue, GFP-Delta 2+ in green and antibody staining in red. In merged images `yellow' indicates spectral overlap between red and green images. Bar, 10 µm.