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Fig. 2. Conservation of the clathrin and adaptin from T. brucei. The evolutionary relationship of TbCLH and TbAPß1 was analysed using PAUP. TbCLH is demonstrated to be most closely related to the heavy chain of S. cerevisiae (A) and TbAPß1 is most homologous to S. cerevisiae ß1-adaptin (B). Numbers represent the bootstrap percent confidence for various branch points and horizontal distances represent relative genetic distance. Vertical distances are for clarity only. The ß-adaptin sequences used are: Human (Hs) APß1, ß2, ß3, ß4; Yeast (Sc) APß1 (apl2p), ß2 (apl1p), ß3 (apl6p); A. thaliana (At APß); and T. brucei ß1 (Tb APß1).