Fig. 5. Localisation of TbCLH and TbAPß1 in different life stages. The far-left panels show PCF cells (A,C) or BSF cells (B,D) probed with anti-TbCLH (A,B) or anti-TbAPß1 antibodies (C,D). The centre-left panels show DAPI staining, and the centre-right panels show phase-contrast of the cells. The position of the antibody labelling relative to the nucleus and the kinetoplast is shown in the merged panels (far-right). In PCF, TbCLH is localised to discrete punctata between the nucleus and the kinetoplast but is widely distributed on large membraneous structures throughout the posterior end of the BSF. In the PCF, TbAPß1 is present on several small peri-nuclear vesicles and reticular elements (C) but, by contrast, is concentrated in two large perinuclear structures in the BSF (D).