Fig. 6. TbCLH and TbAPß1 closely associate with the Golgi complex, but are on distinct membranes. (A,B) BSF cells were stained with TbCLH (A, left) or TbAPß1 (B, left), BODIPY-ceramide (middle), and DAPI (in all panels); the merged image is shown on the right. TbCLH is observed in structures juxtaposed to the Golgi complex, whereas the TbAPß1 membranous structure nearest to the nucleus partially co-localises to the Golgi complex as seen in yellow in the merged image. (C,D) PCF (C) and BSF (D) cells labelled with rabbit anti-TbCLH (red) and mouse anti-TbAPß1 (green) and examined by confocal microscopy. The merged image is shown on the right; the asterix indicates the position of the nucleus. In neither of these life stages is there significant coincidence.