Fig. 9. Ultra thin cryosections showing subcellular localisation of TbCLH. (A) Conventional Epon section showing clathrin-like coats on a vesicle (arrowheads) and a tubular profile (arrows). (B-D) Cryosections labelled with TbCLH followed by 6 nm protein A gold show clathrin in association with a tubular profile (B); on membranes and vesicles in a region between the Golgi and the flagellar pocket (C); and also TbCLH labelling of membranes and vesicles just below the plasma membrane (D). In C, the 6 nm gold particles originally used for increased resolution have been digitally enhanced using Adobe Illustrator by placing black circles corresponding to 15nm diameter over the Gold particles. Bars, 100 nm, the position of the Golgi complex, endoplasmic reticulum (ER) and plasma membrane (PM) are indicated.