Fig. 4. TbRAB11 migrates coordinately with the basal body during mitosis. PCF parasites were treated for microscopy and stained with mouse anti-basal body (BB4) followed by an anti-mouse FITC conjugate (green), rabbit anti-TbRAB11 followed by an anti-rabbit Cy3 conjugate (red) and with DAPI for DNA (blue). For each image the basal body stain is shown to the left, the central figure corresponds to TbRAB11 and the right image is the merge. (A-C) Cells prior to entering mitosis with one kinetoplast and one nucleus. (D-F) Cells with one nucleus and an elongate duplicating kinetoplast and basal body. (G-I) Cells in which the kinetoplast and basal body have both duplicated while there is still a single nucleus.