Fig. 5. TbRAB11 is absent from lysosomal and Golgi elements. (A-D) BSF parasites were treated for fluorescent microscopy and stained with mouse anti-p67 followed by an anti-mouse FITC conjugate (green), rabbit anti-TbRAB11 followed by an anti-rabbit Cy3 conjugate (red) and DAPI for DNA (blue). (E-H) BSF parasites were exposed to biotinylated ConA at 4°C for 10 minutes prior to chase for 30 minutes at 37°C to permit lysosomal ConA accumulation. Cells were then treated for microscopy and stained with FITC-avidin (green), rabbit anti-TbRAB11 followed by an anti-rabbit Cy3 conjugate (red) and DAPI for DNA (blue). (I-L) BSF parasites were treated with BODIPY-TR ceramide (red) to stain the Golgi (Field et al., 2000). Cells were subsequently processed for microscopy and counterstained with rabbit anti-TbRAB11 followed by an anti-rabbit Oregon Green conjugate (green) and DAPI for DNA (blue).